The smart Trick of Pos88 That No One is Discussing
The smart Trick of Pos88 That No One is Discussing
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To study the effect of multiple constraints on mutational tolerance in the course of collection, we selected E. coli dihydrofolate reductase (DHFR) as a design method. DHFR is An important enzyme within just folate metabolism that lowers dihydrofolate to tetrahydrofolate which is necessary for thymidine creation. making use of this action as The idea for an in vivo choice assay (Reynolds et al., 2011), we aimed first to evaluate a mutational landscape for DHFR and after that to determine how a improve into the mobile ecosystem might impact the landscape. mainly because DHFR is understood to progress by many conformational states during catalysis (Boehr et al.
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